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Quality Control Data Sheet

A010-12    anti-phospho-PLB (Ser-16)    lot:0401-02

Immunogen: Synthetic peptide coupled to Keyhole limpet hemocyanin

 

Formulation: Whole lyophilised rabbit serum.

 

Antigen/specificity: Polyclonal antibody raised to the phosphopeptide sequence RSAIRRAS(PO4)TIEY. Recognises Ser-16 phosphorylated PLB exclusively, and will not react with dephosphorylated or dual (Ser-16/Thr-17) phosphorylated PLB.

 

Reconstitution & Storage: Store dessicated at 4C prior to reconstitution. Dissolve lyophilised serum in 20ml deionised water. Freeze in aliquots at –70C. Stable for at least 12 months. Avoid freeze-thaw cycles - this denatures antibody molecules.

 

Species reactivity: Bovine, dog, ferret, hamster, human, rat, sheep

Applications: Western blot (1:5000), immunofluorescence microscopy (1:200)

 

 

 

Quality control testing

Immunoblot analysis: PS-16 (1:5000), of this lot, detected Ser-16 phospho-PLB in 0.3mg cardiac SR (figure 1) and rat heart homogenate (100mg). All immunoreactivity was absent when blots were probed by PS-16 in the presence of 1mM epitope peptide (P010-16), data not shown.

 

 

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Last modified: July 26, 2005