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Quality Control Data Sheet

A010-30    anti-phospho-RYR2 (Ser-2809), RYR2-PS2809    lot:0401-04

Immunogen: Synthetic peptide coupled to Keyhole limpet hemocyanin

 

Formulation: Whole lyophilised rabbit serum.

 

Antigen/specificity: Polyclonal antibody raised to the phosphopeptide sequence YNRTRRIS(PO4)QT. Recognises Ser-2809 phosphorylated RYR2 exclusively, and will not react with dephosphorylated RYR2.

 

Reconstitution & Storage: Store dessicated at 4C prior to reconstitution. Dissolve lyophilised serum in 50ml deionised water. Freeze in aliquots at –70C. Stable for at least 12 months. Avoid freeze-thaw cycles - this denatures antibody molecules.

 

Species reactivity: Dog,  rat, sheep

  Applications: Western blot 1:5000 immunofluorescence 1:200

 

 

 

Quality control testing

Immunoblot analysis: RYR2-PS2809 (1:5000), of this lot, detected Ser-2809 phospho-RYR2 in 10mg cardiac SR (figure). All immunoreactivity was absent when blots were probed by RYR2-PS2809 in the presence of 1mM epitope peptide (P010-30), but addition of 1mM dephosphorylated peptide (P010-35) had no effect on antibody binding to phosphorylated RYR2.

 

 

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Last modified: July 26, 2005