Badrilla
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Quality Control Certificate of analysis Lot 0503-01 anti-phospho-PLB (Thr-17) Immunogen:
Synthetic peptide conjugated to keyhole limpet haemocyanin Formulation:
Lyophilised rabbit serum, containing rabbit IgG specific for immunogen Antigen
specificity:
Polyclonal antibody raised to the phosphopeptide sequence
RSAIRRAST(PO4)IEY. Recognises Thr-17 phosphorylated PLB, and
will not react with dephosphorylated PLB or Ser-16 phosphorylated PLB. Reconstitution
& Storage:
Store dessicated at 4C prior to reconstitution.
Dissolve lyophilised serum in 20ml deionised water.
Freeze in aliquots at –70C. Avoid
freeze-thaw cycles - this denatures
antibody molecules. Best Before Date: This antibody preparation stored as described on
this sheet is very stable. It will display the characteristics described on this
sheet if used before 31 May 2004. Species
reactivity:
Dog, rat. [From previous
experience, it is likely that this product will also detect Thr-17
phosphorylated forms of PLB in human, ferret, guinea pig and mouse samples of
cardiac muscle although evidence for this has not been sought for this batch of
antibody.] Quality control testing Immunoblot analysis: anti-phospho-PLB (Thr-17) (1:5000), of this
lot, detected Thr-17 phosphorylated PLB in 0.8mg
canine cardiac SR (lane c) but not Ser-16 phosphorylated PLB in the same SR
preparation (lane b). It also detected Thr-17 phosphorylated PLB in rat heart
homogenates (25mg,
lane a). All specific immunoreactivity was absent when blots were probed by
antibody in the presence of 1mM epitope peptide (P010-13), but addition of 1mM
epitope peptide to the Ser-16 site (P010-12) had no effect on antibody binding
to phosphorylated PLB. It should be noted that a non-specific interaction
of the serum with a protein of ~30kDa was observed in rat heart homogenates (*).
Researchers are advised to perform experiments using epitope peptides to
establish specific and non-specific interactions in their samples.
Date: 30th
May 2003
Signed: Name: J.
Colyer, Ph.D. Proprietor
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