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Anti-phospholamban antibody A1

Catalog No. Unit Volume Price  
A010-14 20 μg £125 or $295US or €210 place an order

Description: Murine monoclonal antibody specific to phospholamban.

Antibody form: Lyophilised affinity purified IgG.

Species recognition: All mammalian species, oligomeric and monomeric PLB.

Applications: Western blot (1:5000 dilution)
Immunofluorescence microscopy (5μg/ml)
Ca2+ pump assay formats (2μg/μg SR)

Storage: 4°C lyophilised; -80°C reconstituted

Further information:
Product datasheet (68KB PDF file, opens in a new window)
MSDS certificate (25KB PDF file, opens in a new window)

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Related products:
Anti- PLB PS-16
Anti-PLB PT-17

References:
Jackson, W.A. & Colyer, J. (1996) Biochem. J. 316, 201-207.
Morris, G.L. et al. (1991) J. Biol. Chem. 266, 11270-11275.
Suzuki, T. & Wang, J.H. (1986) J. Biol. Chem. 261, 7018-7023.

Epitope sequence alignment

Epitope LTRSAIRRAS
Human LTRSAIRRAS
Bovine LTRSAIRRAS
Dog LTRSAIRRAS
Mouse LTRSAIRRAS
Pig LTRSAIRRAS
Rabbit LTRSAIRRAS
Rat LTRSAIRRAS
Chick ITRSALRRAS



Figure 1: Immunodetection of phospholamban in rat heart homogenate
(A) 32P-phosphoproteins in rat heart homogenate showing PLB (arrows) on autoradiograph. (B) A1 detection of PLB in rat heart homogenate (100μg): (lanes 1,2)cAMP, (lanes 3,4) Ca2+/CaM. Samples in lanes 2,4 were boiled for 5 minutes prior to SDS-PAGE. Adapted from Drago & Colyer (1994) J. Biol. Chem. 271, 25073-25077.