Anti-RyR2 phospho-Ser2814 antibody

Catalog No.

Unit Volume

Price

A010-31

50μl

£248 or $570US or €413

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Description: Polyclonal antibody specific to Ser-2814 phosphorylated RYR2.

Antibody form: Lyophilised rabbit anti-serum (IgG).

Species recognition: all mammalian species, when phosphorylated on Ser-2814 (or Ser-2815 rabbit) Antibody recognises epitope despite single residue difference in epitope between species (V2815I; human vs mouse).

Applications: Western blot (1:1000 dilution), immunofluorescence microscopy (1:100)

Storage: 4°C lyophilised; -80°C reconstituted

Further information:
Product datasheet (417KB PDF file, opens in a new window)
MSDS certificate (26KB PDF file, opens in a new window)

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References:
Carter, S., Colyer, J., & Sitsapesan, R. (2006) Circ. Res. 98, 1506-1513.
Rodriguez, P., Bhogal, M. S., and Colyer, J. (2003) J. Biol. Chem. 278, 38593-600.

Figure 1: Detection of RYR2 phosphorylated at Ser2814 in rat cardiac SR
Cardiac SR vesicles were phosphorylated by CaMKII (sample b) or PKA (sample c) for 1 min at 37C. Control vesicles were incubated without kinase or ATP (a). 10μg protein was separated by 6% SDS-PAGE electrophoresis and transferred to PVDF membraneSer-2814 phosphorylated RYR2 was detected with anti-RYR2 phospho-Ser2814 (A010-31: 1:1000) detected with peroxidise based chemiluminescence. Similar results were obtained with 1:2000 dilution of A010-31. Epitope peptide (P010-31, 1μM) abolished all immunorecognition, indicating that all staining is specific for phospho-2814 RYR2. The position of molecular weight markers at 175kDa, 83kDa, 66kDa and 45kDa are marked on the figure.