Anti-RyR2 Dephos-Ser2808 antibody

Catalog No.

Unit Volume

Price

A010-35

50μl

£248 or $570US or €413

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Description: Polyclonal antibody specific to Ser-2808 dephosphorylated RYR2.

Antibody form: Lyophilised rabbit anti-serum (IgG).

Species recognition: all mammalian species, when dephosphorylated on Ser-2808 (or 2809 rabbit)

Applications: Western blot (1:5000 dilution), immunofluorescence microscopy (1:100)

Storage: 4°C lyophilised; -80°C reconstituted

Further information:
Product datasheet (59KB PDF file, opens in a new window)
MSDS certificate (26KB PDF file, opens in a new window)

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References:
Carter, S., Colyer, J., & Sitsapesan, R. (2006) Circ. Res. 98, 1506-1513.
Rodriguez, P., Bhogal, M. S., and Colyer, J. (2003): J. Biol. Chem. 278, 38593-600.

Figure 1: Immunoblot analysis of RYR2 phosphorylation and dephosphorylation
Canine cardiac SR vesicles were incubated with CaMKII (30C, 1min) in absence (lane a) and presence (lane b) of MgATP. Samples (10μg) was separated using 6% SDS-PAGE gels and transferred to PVDF. RYR2 protein was detected using antibodies 34C (Total), anti-RYR2 phospho-Ser2808 (A010-30, 1:5000: phos-) or anti-RYR2 dephos-Ser2808 (A010-35, 1:5000: dephos-). Immunoreactive proteins were detected using peroxidise based chemiluminescence.