Anti-Cav1.2 phospho-Ser1928 antibody

Catalog No.

Unit Volume

Price

A010-70

50μl

£248 or $570US or €413

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Description: Polyclonal antibody specific to Ser-1928 phosphorylated Cav1.2 (α1c sub-unit of L-type Ca²⁺-channel).

Antibody form: Lyophilised rabbit anti-serum (IgG).

Species recognition: all mammalian species, when phosphorylated on Ser-1928.

Applications: Immunofluorescence microscopy (1:100)

Storage: 4°C lyophilised; -80°C reconstituted

Further information:
Product datasheet (505KB PDF file, opens in a new window)
MSDS certificate (26KB PDF file, opens in a new window)
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References:
De Jongh, K.S., Murphy, B.J., Colvin, A.A., Hell, J.W., Takahashi, M. & Catterall, W.A. (1996) Biochemistry 35, 10392-402.

Figure 1: Detection of Cav1.2 phosphorylated at Ser1928 in rat cardiac myocytes
Rat cardiac myocytes were stimulated electrically (0.5Hz) and with β1-adrenergic agonist (100nM isoproterenol + 100nM ICI118551) for 5 minutes. Cells were fixed in 4% formaldehyde for 30min, washed in PBS (3 times) and permeabilised in 0.1% Triton X-100 in PBS. Non-specific binding sites were blocked with donkey serum, and cells were incubated with anti-Cav1.2 phospho-Ser1928 antibody (A010-70) at 1:100 dilution in the absence (top) or presence of 3μM epitope peptide (P010-70: bottom) for 60min at room temperature. Cells were washed 3 times in PBS and incubated with fluorescently labelled secondary antibody (Alexa Fluor donkey anti-rabbit IgG, 1:500) for 2 hours. Cells were washed (3xPBS) and mounted on a slide and viewed under a confocal fluorescence microscope under oil immersion.