Badrilla
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Catalogue No.: P010-50 Unit Size: 50 nmoles; sufficient for 50 mls of western blot reagent at 1 μM Price: £68 / €114 / US$136 Place an order Background: Calcium/calmodulin-dependent protein kinase II (CaMKII) is a ubiquitous, multifunctional serine/threonine kinase involved in various calcium regulated cellular functions (Schulman and Braun, 1999). The multimeric protein is composed of 8 -12 individual protein kinase subunits, which are the products of four separate genes, α, β, γ, δ, and are expressed in a tissue-specific manner (Schulman and Braun, 1999). Subunit phosphorylation at Thr-286 (or 287 depending on isoform) results in an autonomous Ca2+/CaM independent activity and in an increased Ca2+/CaM binding affinity. This allows for CaMKII to retain maximal catalytic activity for prolonged times at low Ca2+ concentrations and partial activity in the absence of bound Ca2+/CaM (Braun and Schulman, 1995). Description: Synthetic competing epitope peptide containing amino acids surrounding the phosphorylated threonine residue at position 286 (or 287) of CaMKII. Peptide Sequence: NH2-GG-K(5 Fam)-YM281HRQET(PO3H2)VDC289-CONH2 M. W. = 1959.9 Da. Sequence in bold = native epitope sequence. Specificity: Addition of the peptide to the primary antibody incubation at a 1 μM final concentration is sufficient to prevent the recognition of the anti-CaMKII phospho-Thr286 antibody to its target epitope (see Quality Control Certificate for the anti-CaMKII phospho-Thr286 antibody).
Quality Control Certificate of Analysis
Related Products: Anti-CaMKII phospho-Thr286 antibody (A010-50). Background References: • Braun, A. P., and Schulman, H. (1995): Annu Rev Physiol 57, 417-45. • Schulman, H., and Braun, A. P. (1999): In E. Carafoli, and C. Klee (Eds): Calcium as a Cellular Regulator, Oxford University Express, New York. |
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