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Anti-Phospholamban Phospho-Ser16 Antibody

Catalogue No.: A010-12           Unit Size: 20 μl; sufficient for 100 mls of western blot reagent     

Price: £125 / €210 / US$295     Place an order

Background: Phospholamban (PLB) is a small transmembrane protein which plays an important role in controlling the activity of the sarcoplasmic reticulum ATPase (SERCA2a) of cardiac muscle during calcium sequestration (Drago and Colyer, 1994). Phospholamban is phosphorylated on separate amino acid residues by cAMP-dependent (Ser-16, Simmerman et al., 1986) and Ca²⁺/CaM-dependent (Thr-17, Simmerman et al., 1986) protein kinases in response to β-adrenergic stimulation (Wegener et al., 1989). The result is an increased calcium pump activity which reduces the calcium transient, increases the calcium load in the sarcoplasmic reticulum, and consequently, produces a larger calcium transient at the next action potential (Sham et al., 1991). PLB reversibly inhibits SERCA2a in order to control its activity. However, alteration in this homeostatic interaction has been shown to result in heart failure (MacLennan and Kranias, 2003).

Description: 20 μl of lyophilised rabbit polyclonal serum containing IgG antibody specific to PLB phospho-Ser16.

Immunogen: Synthetic peptide (R₉SAIRRAS(PO3H2)TIEY₂₀) corresponding to amino acids surrounding the phosphorylated serine residue at position 16 of PLB.

Specificity and Species Cross Reactivity: The antibody recognises serine 16 of mono- and oligomeric phospholamban when phosphorylated by PKA. Binding of the antibody to its target epitope is blocked in the presence of a phosphopeptide containing the PLB phospho-Ser16 epitope. Antibody affinity is reduced in circumstances of dual phosphorylation of Ser-16 and Thr-17. The antibody reacts with phospho-Ser16 of phospholamban from bovine, canine, ferret, hamster, human, rat and sheep species.

Quality Control Certificate of Analysis                                        Western Blot Protocol

                                                           Quality Control Certificate of Analysis for lot: 0401-02

Related Products: PLB phospho-Ser16 epitope peptide (P010-12); Anti-PLB phospho-Thr17 antibody (A010-13); Anti-PLB A1 antibody (A010-14).

Background References:                                                          

• Drago, G. A., and Colyer, J. (1994): J Biol Chem 269, 25073-7.                                                                                                  

• MacLennan, D. H., and Kranias, E. G. (2003): Nat Rev Mol Cell Biol 4, 566-77.                                                                   

• Sham, J. S., Jones, L. R., and Morad, M. (1991): Am J Physiol 261, H1344-9.                                                                             

• Simmerman, H. K., Collins, J. H., Theibert, J. L., Wegener, A. D., and Jones, L. R. (1986): J Biol Chem 261, 13333-41.            

• Wegener, A. D., Simmerman, H. K., Lindemann, J. P., and Jones, L. R. (1989): J Biol Chem 264, 11468-74.

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Last modified: November 14, 2007