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Anti-RyR2 Dephos-Ser2809 Antibody

Catalogue No.: A010-35           Unit Size: 50 μl; sufficient for 250 mls of western blot reagent     

Price: £248 / €413 / US$570     Place an order

Background: The ryanodine receptor (RyR2) is a Ca²⁺ channel of cardiac muscle that plays a central role in EC coupling. The binding of Ca²⁺ to RyR2 opens the channel and Ca²⁺ stored in the SR moves through the channel into the cytosol to initiate muscle contraction (Bers, 2002). Abnormal structure and function of ryanodine receptors has been reported in failing hearts, with Ser-2809 phosphorylation appearing elevated in clinical situations which may contribute to the abnormal Ca²⁺ handling characteristics of cardiac muscle in these conditions (Wehrens and Marks, 2003). Ser-2809 can be phosphorylated by PKA or CaMKII (Rodriguez et al., 2003), which is coincident with significant change in RyR2 channel function typified by an increased open probability (Witcher et al., 1991; Valdivia et al., 1995; Marx et al., 2000), the abrogation of the inhibitory effects of CaM (Witcher et al., 1991) and Mg²⁺ (Hain et al., 1995), dissociation of regulatory factors, expression of subconductance states and the expression of channel activity at diastolic Ca²⁺ concentrations (Marx et al., 2000).

Description: 50 μl of lyophilised rabbit polyclonal serum containing IgG antibody specific to RyR2 dephos-Ser2809.

Immunogen: Synthetic peptide (R₉SAIRRAS(PO3H2)TIEY₂₀) corresponding to amino acids surrounding the phosphorylated serine residue at position 16 of PLB.

Specificity and Species Cross Reactivity: The antibody recognises dephosphorylated serine 2809 of the ryanodine receptor. Binding of the antibody to its target epitope is blocked in the presence of a phosphopeptide containing the dephos-Ser2809 epitope. The antibody reacts with dephos-Ser2809 of phospholamban from canine, rat and sheep species.

Quality Control Certificate of Analysis                                        Western Blot Protocol

                                                           Quality Control Certificate of Analysis for lot: 0401-05

Related Products: RyR2 dephos-Ser2809 epitope peptide (P010-35); Anti-RyR2 phospho-Ser2809 antibody (A010-30).

Background References:                                                          

• Bers, D. M. (2002): Nature 415, 198-205.                         

• Hain, J., Onoue, H., Mayrleitner, M., Fleischer, S., and Schindler, H. (1995): J Biol Chem 270, 2074-81.               

• Marx, S. O., Reiken, S., Hisamatsu, Y., Jayaraman, T., Burkhoff, D., Rosemblit, N., and Marks, A. R. (2000): Cell 101, 365-76.

• Rodriguez, P., Bhogal, M. S., and Colyer, J. (2003): J Biol Chem 278, 38593-600.                                                             

• Valdivia, H. H., Kaplan, J. H., Ellis-Davies, G. C., and Lederer, W. J. (1995): Science 267, 1997-2000.             

• Wehrens, X. H., and Marks, A. R. (2003): Trends Biochem Sci 28, 671-8.                                                                            

• Witcher, D. R., Kovacs, R. J., Schulman, H., Cefali, D. C., and Jones, L. R. (1991): J Biol Chem 266, 11144-52.

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Last modified: November 14, 2007