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Anti-CaMKII Phospho-Thr286 Antibody
Catalogue No.: A010-50 Unit Size: 20 μl; sufficient for 100 mls of western blot reagent Price: £125 / €210 / US$295 Place an order Background: Calcium/calmodulin-dependent protein kinase II (CaMKII) is a ubiquitous, multifunctional serine/threonine kinase involved in various calcium regulated cellular functions (Schulman and Braun, 1999). The multimeric protein is composed of 8 -12 individual protein kinase subunits, which are the products of four separate genes, α, β, γ, δ, and are expressed in a tissue-specific manner (Schulman and Braun, 1999). Subunit phosphorylation at Thr-286 (or 287 depending on isoform) results in an autonomous Ca2+/CaM independent activity and in an increased Ca2+/CaM binding affinity. This allows for CaMKII to retain maximal catalytic activity for prolonged times at low Ca2+ concentrations and partial activity in the absence of bound Ca2+/CaM (Braun and Schulman, 1995). Description: 20 μl of lyophilised rabbit polyclonal serum containing IgG antibody specific to CaMKII phospho-Thr286. Immunogen: Synthetic peptide (M281HRQET(PO3H2)VDC289) corresponding to amino acids surrounding the phosphorylated threonine residue at position 286 of CaMKII. Specificity and Species Cross Reactivity: Specific for CamKII phospho-Thr286 (or 287) α, β, γ and δ isoforms in rat, human and mouse. Expected to react with various isoforms from chicken, cow, pig, rabbit, orangutan, zebrafish, ferret and Xenopus laevis (see Quality Control Certificate for more information).
Quality Control Certificate of Analysis Western Blot Protocol
Related Products: CaMKII phospho-Thr286 epitope peptide (P010-50). Background References: • Braun, A. P., and Schulman, H. (1995): Annu Rev Physiol 57, 417-45. • Schulman, H., and Braun, A. P. (1999): In E. Carafoli, and C. Klee (Eds): Calcium as a Cellular Regulator, Oxford University Express, New York. |
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