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anti-phospholamban antibody A1

Catalog A010-14        Unit size 20mg         Unit price £125 or $295US or €210       to place an order

Monoclonal antibody (A1) raised against bovine cardiac PLB1. Recognises an epitope comprising residues 7LTRSAIRRAS162 and reacts with all forms of PLB, monomer, oligomers, phosphorylated, non-phosphorylated (figure 1). Stimulates Ca2+-pump activity upon binding PLB, to an extent equivalent to stoichiometric phosphorylation of PLB (figure 2)3. Recognises PLB from bovine, canine, ferret, hamster, human, rat and sheep species. Useful in ELISA, Western Blot (0.2mg/ml), immunoprecipitation, immunomicroscopy (5mg/ml) and Ca2+-pump assay formats (2mg/mg SR). Antibody presented as affinity purified immunoglobulin (protein G chromatography).

references: 1. Suzuki & Wang (1986) J. Biol. Chem. 261, 7018-7023: 2. Morris et al. (1991) J. Biol. Chem. 266, 11270-11275: 3. Jackson & Colyer (1996) Biochem. J. 316, 201-207

                                         

                   

Quality Control data files    QC0401-03

Related products    epitope peptide (A1)

                           anti-phospho-PLB (Ser-16)

                           anti-phospho-PLB (Thr-17)     

Experimental Protocols        western blot protocol

 

 

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Last modified: November 14, 2007