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anti-phospholamban antibody A1 Catalog A010-14 Unit size 20mg Unit price £125 or $295US or €210 to place an order Monoclonal antibody (A1) raised against bovine cardiac PLB1. Recognises an epitope comprising residues 7LTRSAIRRAS162 and reacts with all forms of PLB, monomer, oligomers, phosphorylated, non-phosphorylated (figure 1). Stimulates Ca2+-pump activity upon binding PLB, to an extent equivalent to stoichiometric phosphorylation of PLB (figure 2)3. Recognises PLB from bovine, canine, ferret, hamster, human, rat and sheep species. Useful in ELISA, Western Blot (0.2mg/ml), immunoprecipitation, immunomicroscopy (5mg/ml) and Ca2+-pump assay formats (2mg/mg SR). Antibody presented as affinity purified immunoglobulin (protein G chromatography). references: 1. Suzuki & Wang (1986) J. Biol. Chem. 261, 7018-7023: 2. Morris et al. (1991) J. Biol. Chem. 266, 11270-11275: 3. Jackson & Colyer (1996) Biochem. J. 316, 201-207
Quality Control data files QC0401-03 Related products epitope peptide (A1) Experimental Protocols western blot protocol |
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