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Anti-SERCA2 Phospho-Ser38 Antibody

Catalogue No.: A010-25           Unit Size: 20 μl; sufficient for 100 mls of western blot reagent    (Package includes positive control protein)

Price: £125 / €210 / US$295     Place an order

Background: SERCA2 belongs to the P-type family of ATPases (Xu et al., 1993; Toyofuku et al., 1994; Hawkins et al., 1994; Osada et al., 1998; Netticadan et al., 1999; Netticadan et al., 2000). Controversy still surrounds the phosphorylation status of Ser-38 and its physiological implication. Some groups have reported that Ser-38 of SERCA2 is phosphorylated by CaMKII, which apparently leads to a substantial increase in ATPase activity (Xu et al., 1993; Hawkins et al., 1994). However, the control of Ca²⁺-pump function by direct phosphorylation has not been observed by all investigators (Odermatt et al., 1996; Reddy et al., 1996; Rodriguez et al., 2004). This antibody, described by Rodriguez et al. (2004), may help to resolve the controversy.

Description: 20 μl of lyophilised rabbit polyclonal serum containing IgG antibody specific to SERCA2 phospho-Ser38.

Immunogen: Synthetic peptide (K₃₁LKERWGS(PO3H2)NEL₄₁) corresponding to amino acids surrounding the phosphorylated  serine residue at position 38 of SERCA2.

Specificity and Species Cross Reactivity: The antibody recognises the phospho-Ser38 epitope within a peptide, which is part of a positive control protein (see Quality Certificate for SERCA2 Phospho-Ser38 Positive Control) since Ser-38 has yet to be shown to be phosphorylated in canine cardiac SR samples (Rodriguez et al., 2004). Reacts with dog. Not yet tested in other species but is expected to react with human, rat, mouse, rabbit and cat due to 100% identical epitope sequences.

 Quality Control Certificate of Analysis                    Western Blot Protocol

Related Products: SERCA2 phospho-Ser38 epitope peptide (P010-25). SERCA2 phospho-Ser38 positive control (C010-25).

Background References:                                                          

• Hawkins, C., Xu, A., and Narayanan, N. (1994): J Biol Chem 269, 31198-206.                                                                           

• Netticadan, T., Temsah, R., Osada, M., and Dhalla, N. S. (1999): Am J Physiol 277, C384-91.                                            

• Netticadan, T., Temsah, R. M., Kawabata, K., and Dhalla, N. S. (2000): Circ Res 86, 596-605.                                                 

• Odermatt, A., Kurzydlowski, K., and MacLennan, D. H. (1996): J Biol Chem 271, 14206-13.                                                                       

• Osada, M., Netticadan, T., Tamura, K., and Dhalla, N. S. (1998): Am J Physiol 274, H2025-34.                                          

• Reddy, L. G., Jones, L. R., Pace, R. C., and Stokes, D. L. (1996): J Biol Chem 271, 14964-70.                                            

• Rodriguez, P., Jackson, W. A., and Colyer, J. (2004): J Biol Chem 279, 17111-9.                                                                    

• Toyofuku, T., Curotto Kurzydlowski, K., Narayanan, N., and MacLennan, D. H. (1994): J Biol Chem 269, 26492-6.                

• Xu, A., Hawkins, C., and Narayanan, N. (1993): J Biol Chem 268, 8394-7.

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Last modified: November 14, 2007