Badrilla
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HPLC chromatogram showing the purification of the SERCA2 phospho-Ser38 epitope peptide on a C18 column. HPLC gradient = H2O + 0.1% TFA (A) vs. CH3CN + 0.1% TFA (B); isocratic gradient of 20% B over 17 minutes. Shaded area represents the fractions collected and used.
Electrospray mass spectrometry spectrum detailing the purity level of the SERCA2 phospho-Ser38 epitope peptide. |
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